Local pH Gradient Initiated by Light on TiO 2  for Light‐Triggered Modulation of Polyhistidine‐Tagged Proteins | Zendy

Andreeva Daria V. | Zendy; Melnyk Inga | Zendy; Baidukova Olga | Zendy; Skorb Ekaterina V. | Zendy

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Local pH Gradient Initiated by Light on TiO 2 for Light‐Triggered Modulation of Polyhistidine‐Tagged Proteins

Author(s) -

Andreeva Daria V.,

Melnyk Inga,

Baidukova Olga,

Skorb Ekaterina V.

Publication year - 2016

Publication title -

chemelectrochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.182

H-Index - 59

ISSN - 2196-0216

DOI - 10.1002/celc.201600268

Subject(s) - nitrilotriacetic acid , irradiation , polystyrene sulfonate , chemistry , histidine , desorption , sulfonate , femtosecond , layer (electronics) , adsorption , biochemistry , optics , inorganic chemistry , amino acid , organic chemistry , laser , pedot:pss , chelation , nuclear physics , sodium , physics

A new principle of photo‐assisted spatial desorption of (poly)histidine‐tagged (His‐Tag) proteins on a TiO 2 surface is suggested. A semiconductor TiO 2 surface is decorated by layer‐by‐layer (LbL) assembly of a strong polyelectrolyte, namely, polystyrene sulfonate (PSS), and nickel–nitrilotriacetic acid (NTA). The PSS/NTA multilayer architecture provides n ‐fold ( n ×NTA) binding efficiency for more precise protein recognition in comparison to existing molecular His‐Tag protein recognition with one‐ and threefold multiplication (1×NTA, 3×NTA). Spatially resolved desorption of proteins is regulated by non‐ photodestructive short‐term low‐intensity light irradiation (<5 mW cm −2 ). The local pH shift on irradiated TiO 2 selectively affects the pH‐sensitive NTA/protein complex, but not the LbL assembly of PSS and NTA, which is stable in a broad pH range.

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