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Attaching Redox Proteins onto Electrode Surfaces by using bis‐Silane
Author(s) -
Trashin Stanislav,
de Jong Mats,
Meynen Vera,
Dewilde Sylvia,
De Wael Karolien
Publication year - 2016
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201600021
Subject(s) - redox , electrode , chemistry , electron transfer , silane , mesoporous material , kinetics , inorganic chemistry , chemical engineering , photochemistry , organic chemistry , catalysis , physics , quantum mechanics , engineering
Immobilization of redox proteins on electrode surfaces is of special interest for mechanistic studies and applications, because of the well‐controlled redox state of protein molecules at a polarized electrode and fast electron‐transfer kinetics that are free from diffusion limitation. Here, bis‐organosilane [1,2‐bis(trimethoxysilyl)ethane] was used as a fresh solution in a pH 7 phosphate buffer without the use of any organic solvent, sol‐gel, or mesoporous bulk matrix. A short aging period of 30 min before deposition on the electrodes was optimal for the immobilization of proteins. Three redox proteins (cytochrome c, neuroglobin, and GLB‐12) were confined to the gold surface of electrodes with high coverages and stabilities, indicating that the suggested technique is simple, efficient, and generic in nature.