Premium
Electron Transport through Early Exponential‐Phase Anode‐Grown Geobacter sulfurreducens Biofilms
Author(s) -
StrycharzGlaven Sarah M.,
Roy Jared,
Boyd Darryl,
Snider Rachel,
Erickson Jeffrey S.,
Tender Leonard M.
Publication year - 2014
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201402168
Subject(s) - geobacter sulfurreducens , electron transport chain , anode , extracellular , phase (matter) , redox , microelectrode , electrode , biofilm , geobacter , chemistry , materials science , cathode , biophysics , analytical chemistry (journal) , chemical engineering , chemical physics , inorganic chemistry , biology , bacteria , biochemistry , chromatography , organic chemistry , engineering , genetics
Geobacter sulfurreducens biofilms were grown to early exponential phase (i.e. the point at which the catalytic current first begins to increase) on interdigitated microelectrode arrays (IDAs), resulting in the formation of sparse cell clusters surrounded by extracellular polymeric substance (EPS). Continuous domains of EPS (defined here to include extracellular materials including filaments), but not cell clusters, were observed to bridge the gaps between adjacent electrode bands. Electrochemical gate measurements revealed electrical continuity between adjacent IDA electrode bands, indicating that extracellular electron transport (EET) occurs through the EPS. The dependency of the source‐drain current on the gate potential is peak shaped, which is consistent with EPS acting as a redox conductor. The gate potential at which the maximum source‐drain current occurs is approximately 0.13 V more positive than that for stationary‐phase biofilms, indicating that redox cofactors involved in EET at the early exponential phase are different to those at the stationary phase.