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Effect of Multi‐Walled Carbon Nanotubes on Glucose Oxidation by Glucose Oxidase or a Flavin‐Dependent Glucose Dehydrogenase in Redox‐Polymer‐Mediated Enzymatic Fuel Cell Anodes
Author(s) -
Osadebe Isioma,
Leech Dónal
Publication year - 2014
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201402136
Subject(s) - glucose oxidase , redox , chemistry , flavin group , electrode , dehydrogenase , electrochemistry , nuclear chemistry , biosensor , inorganic chemistry , organic chemistry , enzyme , biochemistry
The addition of multi‐walled carbon nanotubes (MWCNTs) to enzymatic electrodes based on either glucose oxidase (GOx), or an oxygen‐insensitive flavin adenine dinucleotide‐dependent glucose dehydrogenase (FADGDH), increases the amount of {Os(4,4′‐dimethyl‐2,2′‐bipyridine) 2 [poly(vinylimidazole)] 10 Cl}Cl redox polymer at the electrode surface, indicating that MWCNTs provide a surface for the immobilisation of film components. Glucose oxidation is highest for films with 68 % ( w / w ) MWCNTs, and a decrease is observed with larger amounts; this decrease is related to a decrease in retained enzyme activity. Enzymatic electrodes provide 4.2 mA cm −2 current density at 0.12 V versus Ag/AgCl, for GOx‐based electrodes, compared to 2.7 mA cm −2 for FADGDH‐based electrodes in 50 m M phosphate‐buffered saline containing 150 m M NaCl at 37 °C. Current densities of 0.52 and 1.1 mA cm −2 are obtained for FADGDH and GOx‐based electrodes, respectively, operating at physiologically relevant 5 m M glucose concentrations. These enzymatic electrodes, thus, show promise for application as anodes in enzymatic fuel cells for in vivo or ex vivo power generation.