Premium
Quinone‐Modified Surfaces for Enhanced Enzyme–Electrode Interactions in Pyrroloquinoline‐Quinone‐Dependent Glucose Dehydrogenase Anodes
Author(s) -
Babanova Sofia,
Matanovic Ivana,
Atanassov Plamen
Publication year - 2014
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201402104
Subject(s) - pyrroloquinoline quinone , electron transfer , redox , chemistry , quinone , electrode , dehydrogenase , anode , benzoquinone , density functional theory , electron transport chain , photochemistry , cofactor , inorganic chemistry , enzyme , organic chemistry , computational chemistry , biochemistry
An approach for enhancing the enzyme–electrode interface reactions with pyrroloquinoline quinone (PQQ)‐dependent glucose dehydrogenase (GDH) is described in this study. Modification of carbonaceous electrodes with ubiquinone or its functional analogues (1,2‐ and 1,4‐benzoquinones) that have the appropriate redox potential to provide a driving force for an electron transfer to occur, along with fast electron‐transfer rate through these molecules, creates an “electron sink” on the electrode surface that can pull electrons from the cofactor, increasing the electron‐transfer rate and generating higher current densities. Several important parameters are experimentally evaluated and/or calculated using density functional theory. Among the quinones investigated, 1,4‐benzoquinone has the greatest influence on the PQQ‐dependent GDH anodes, yielding 5.1‐fold higher current densities on single‐walled, and 3.3‐fold on multi‐walled carbon nanotube papers in comparison to unmodified PQQ‐dependent GDH anodes.