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Biotin‐Labeled Electropolymerized Network of Gold Nanoparticles for Amperometric Immunodetection of Human Fibrinogen
Author(s) -
Díez Paula,
Gamella María,
MartínezRuíz Paloma,
Lanzone Valentina,
Sánchez Alfredo,
Sánchez Enrique,
Garcinuño Belit,
Villalonga Reynaldo,
Pingarrón José M.
Publication year - 2014
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201300114
Subject(s) - amperometry , detection limit , colloidal gold , chemistry , nanoparticle , polymerization , streptavidin , electrode , nuclear chemistry , chromatography , electrochemistry , nanotechnology , biotin , materials science , polymer , biochemistry , organic chemistry
Water‐soluble gold nanoparticles (3.1±0.6 nm) with polymerization ability and affinity to streptavidin are prepared by reducing HAuCl 4 in the presence of the capping ligands 2‐mercaptoethanesulfonic acid, biotin–cysteamine, and p ‐aminothiophenol. This colloid, HAuCl 4 , is used to modify gold electrodes by forming a 3D electropolymerized network of bis‐aniline‐cross‐linked nanoparticles on the metal surface. The modified electrode is used as a scaffold for the assembly of an amperometric immunosensor system to detect human fibrinogen. The immunosensor shows excellent analytical characteristics, with a dynamic range of detection between 0.018 and 2.208 μg mL −1 , a detection limit of 4 ng mL −1 , and a half‐maximal inhibitory concentration ( IC 50 ) of 177 ng mL −1 . The immunosensor is markedly stable, retaining full analytical capacity after 45 days of storage at 4 °C.