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Directly Grown K 0.33 WO 3 Nanosheet Film Electrode for Fast Direct Electron Transfer of Protein
Author(s) -
Liu Jinping,
Liao Lei,
Lu Zhisong,
Yan Bin,
Yu Ting,
Zheng Zhe,
Zhang Jixuan,
Gong Hao,
Huang Xintang,
Li Chang Ming
Publication year - 2014
Publication title -
chemelectrochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 59
ISSN - 2196-0216
DOI - 10.1002/celc.201300013
Subject(s) - nanosheet , electrode , materials science , electron transfer , nanotechnology , electrochemistry , substrate (aquarium) , chemical engineering , analytical chemistry (journal) , chemistry , organic chemistry , engineering , oceanography , geology
A potassium tungstate (K 0.33 WO 3 ) nanosheet film grown directly on a conductive tungsten (W) substrate by a hotplate‐heating approach effects direct electron transfer between the W electrode and a biocatalytic protein or microbe, a long‐sought effect of both fundamental and practical importance. The K 0.33 WO 3 forms into a 5–20 nm thick multilayered nanosheet with a number of steps along the surface. A single nanosheet of K 0.33 WO 3 is hydrophilic and highly electron conducting (resistivity ∼8.3×10 −3 Ω cm), characteristic of metallic behaviour. Glucose oxidase (GOD) immobilized on a K 0.33 WO 3 ‐nanosheet‐coated electrode demonstrates facile direct electron transfer. The electron transfer rate constant ( k s ) is ∼9.5 s −1 . This electrode has been used to construct a direct electrochemistry‐based glucose sensor, which exhibits good sensitivity (as high as ∼66.4 μA m M −1 cm −2 ), fast sensing response time (∼4 s), a low detection limit (0.5 μ M ), high selectivity and good reliability in practical uses. Growing K 0.33 WO 3 nanosheet on electrodes offers a promising general approach for effecting direct bioelectrochemistry for widespread uses in bioelectronic and bioenergy applications.