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Continuous enzyme‐catalyzed production of 6‐aminopenicillanic acid and product concentration by reverse osmosis
Author(s) -
Danzig Joachim,
Tischer Wilhelm,
Wandrey Christian
Publication year - 1995
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.270180406
Subject(s) - penicillin amidase , chemistry , reverse osmosis , chromatography , substrate (aquarium) , hydrolysis , catalysis , precipitation , product inhibition , immobilized enzyme , membrane , organic chemistry , biochemistry , enzyme , oceanography , physics , non competitive inhibition , meteorology , geology
The efficiency of 6‐aminopenicillanic acid (6‐APA) production by enzyme‐catalyzed hydrolysis of penicillin G using penicillin G amidase (E. C. 3.5.1.11) immobilized in polyacrylamide gel can be improved by using a lower substrate concentration and an optimized low buffer concentration. In a continuous process the combination of a stirred‐tank reactor and a plug‐flow reactor gives optimum productivity. Since precipitation of 6‐APA is the crucial step in downstream processing, the product concentration has to be as high as possible. This can be achieved by concentrating the product solution by reverse osmosis.