Premium
Modelling of a fluidized bed bioreactor for immobilized enzymes (Part 2)
Author(s) -
Kiesser Torsten,
Oerstzen Gustav A.,
Bauer Werner
Publication year - 1990
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.270130111
Subject(s) - fluidized bed , bioreactor , immobilized enzyme , mass transfer , chemistry , chromatography , kinetics , continuous stirred tank reactor , chemical engineering , packed bed , thermodynamics , materials science , enzyme , organic chemistry , engineering , physics , quantum mechanics
Abstract In the first part of this contribution, a mathematical model was presented for a liquid fluidized bed using immobilized enzymes, with reversible Michaelis‐Menten kinetics. This part is focused on the experimental results. The reaction kinetics of native and immobilized enzymes was determined in continuous stirred tank reactors under comparable conditions. The influence of external mass transfer was investigated in a fixed bed reactor column. The extend of pore diffusional resistance was examined in a continuous stirred tank reactor and with a numerical simulation. Hydrodynamics was measured in different reactor columns (diameter d t = 0.052 − 0.225 m; length L : 1.0–2.0m) and with a static mixer. Further, the concentration profile was determined in a fluidized bed reactor with side stream analysis for different biocatalyst samples, fluid velocities and bed heights. The simulation of experimental results indicates that they are well described by the developed model. Furthermore, the model is well suited to predicting the influence of specific parameters on the effective kinetics of the biocatalyst and the expansion of the fluidized bed.