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Concomitant Polymorphism and Nucleation Control of DL ‐Methionine Through Antisolvent Crystallization
Author(s) -
Suresh Manivel,
Srinivasan Karuppannan
Publication year - 2021
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.202000300
Subject(s) - supersaturation , nucleation , crystallization , polymorphism (computer science) , chemistry , solubility , metastability , aqueous solution , crystallography , methionine , distilled water , chemical engineering , chromatography , amino acid , organic chemistry , biochemistry , genotype , engineering , gene
Abstract The concomitant polymorphism of the sulfur‐containing essential amino acid DL ‐methionine was revealed, and α ‐ and β ‐polymorphic forms were separated from aqueous solution through antisolvent crystallization with ethanol as antisolvent. The addition of different volume fractions of ethanol into the mother solution reduces the solubility of DL ‐methionine in double‐distilled water and in turn proportionately generates a range of supersaturation levels. This induces the nucleation of a stable β ‐polymorph at low supersaturation levels, a mixture of α ‐ and β ‐ polymorphs at intermediate supersaturation levels, and a metastable α ‐form at high supersaturation levels. Needle‐shaped α ‐polymorphs and platy‐shaped β ‐polymorphs were well distinguished through in‐situ optical microscopic analysis and their structural confirmation was done by X‐ray diffraction analysis.