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Optimization of the Impeller Design for Mesenchymal Stem Cell Culture on Microcarriers in Bioreactors
Author(s) -
Loubière Céline,
Delafosse Angélique,
Guedon Emmanuel,
Toye Dominique,
Chevalot Isabelle,
Olmos Eric
Publication year - 2019
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201900105
Subject(s) - impeller , microcarrier , bioreactor , mesenchymal stem cell , suspension (topology) , computational fluid dynamics , engineering , dissipation , mechanical engineering , mechanics , materials science , chemistry , mathematics , physics , cell , biology , microbiology and biotechnology , thermodynamics , biochemistry , organic chemistry , homotopy , pure mathematics
When agitating mesenchymal stem cells adhered on microcarriers in bioreactors, a compromise has to be found between sufficient particle suspension and limitation of hydromechanical stresses. The present study proposes a strategy to improve the design of an ‘elephant ear' impeller at the just‐suspended state by varying its relative size, blade slope angle, and position in the reactor. To do that, computational fluid dynamics simulations were coupled with multi‐objective optimization to minimize the hydromechanical stress encountered by the microcarriers. Two minimization criteria were considered: ( P/V ) @p and the energy dissipation function EDC . On the basis of 31 conditions, an optimal impeller geometry is proposed.