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Suspension and Mixing Characterization of Intermittent Agitation Modes in DASGIP Bioreactors
Author(s) -
Samaras Jasmin Jade,
Micheletti Martina,
Ducci Andrea
Publication year - 2019
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201900069
Subject(s) - microcarrier , suspension (topology) , bioreactor , mixing (physics) , materials science , porosity , settling , rheology , chemical engineering , chromatography , chemistry , thermodynamics , composite material , mathematics , physics , engineering , cell , organic chemistry , quantum mechanics , homotopy , pure mathematics , biochemistry
Intermittent agitation strategies have been increasingly used for a range of process development applications, i.e., to modulate physical cues, to improve stem cell differentiation yields, and to control hydrodynamic shear stresses in microcarrier suspension; however, there is a distinct lack of characterization. Both continuous and intermittent agitation modes in relation to suspension and mixing dynamics within a DASGIP bioreactor were characterized. Suspension dynamics were found to be affected by microcarrier porosity and the degree of settling was found to be more pronounced at the top of the bioreactor. Mixing time characterization showed a marked improvement in mixing efficiency for intermittent agitation, with an overall dependence on the timing of tracer insertion.