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Immobilization of β ‐Glucosidase BglC on Decanedioic Acid‐Modified Magnetic Nanoparticles
Author(s) -
Wang Nuo,
Wu Rina,
Fu Qiang,
Wang Hongling,
Zhang Zheng,
Haji Ze,
Li Xiutong,
Lian Xinglong,
An Yingfeng
Publication year - 2018
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201700477
Subject(s) - husk , chemistry , thermal stability , magnetic nanoparticles , immobilized enzyme , decomposition , nanoparticle , thermal decomposition , organic chemistry , chemical engineering , nuclear chemistry , enzyme , chromatography , botany , engineering , biology
β ‐Glucosidase BglC from Thermobifida fusca was efficiently immobilized on decanedioic acid‐modified magnetic nanoparticles (DMNP). Interestingly, the immobilized BglC showed much higher organic solvent stability than the free enzyme, indicating that this enzyme immobilized on DMNP should be especially suitable for catalyzing reactions in organic solvents. Additionally, the immobilized BglC also showed better thermal stability, storage stability, and mechanical stability than the free counterpart. Moreover, the reuse times were satisfactory and even crude plant materials (e.g., rice husk and corn cob) were used as substrates, which should be valuable for the decomposition of natural organic materials and the full utilization of bioresources.