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Extended Elution by Characteristic Point Method for Characterization of Protein Ion‐Exchange Adsorption
Author(s) -
Hartig Dave,
Waluga Thomas,
Schmidt Claudia,
Fieg Georg,
Scholl Stephan
Publication year - 2017
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201600560
Subject(s) - elution , adsorption , bovine serum albumin , chemistry , chromatography , ion exchange , hemoglobin , dispersion (optics) , characterization (materials science) , process (computing) , ion , analytical chemistry (journal) , materials science , nanotechnology , computer science , biochemistry , physics , organic chemistry , optics , operating system
Abstract An adequate characterization of adsorption isotherms is mandatory for chromatographic process development. Here, the elution by characteristic point (ECP) is a suitable method with low consumption of material and time. However, the ECP method requires a highly ideal behavior of the measured system. An innovative extended ECP approach is presented to characterize a non‐ideal system by the example of protein ion‐exchange adsorption. A marker, here bovine hemoglobin, is used to quantify all non‐idealities, e.g., convection, dispersion, or low number of theoretical plates. The system signal of the marker is used to compensate the system signal of the interesting solute, here bovine serum albumin. The resulting isotherm agrees well with the control results of a validated static approach.