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Clusters of Phospholipid Vesicles as Platforms for Glucose Oxidase‐Catalyzed Reaction in a Bubble‐Column Bioreactor
Author(s) -
Yoshimoto Makoto,
Sakakida Yuki,
Tamura Ryota,
Natsume Tomotaka,
Ikeda Tomoaki
Publication year - 2016
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201500051
Subject(s) - liposome , glucose oxidase , airlift , chemistry , membrane , catalysis , phospholipid , vesicle , chromatography , oxidase test , biophysics , bioreactor , chemical engineering , biochemistry , biosensor , enzyme , organic chemistry , biology , engineering
Clusters of negatively charged liposomes encapsulated with glucose oxidase were prepared in the presence of Ca 2+ and used to catalyze the oxidation of glucose in an external loop airlift bubble column. The clusters exhibited higher catalytic activity compared to nonclustered glucose oxidase‐containing liposomes (GOLs) when the liposome membranes were incorporated with cholesterol. The clusters were structurally altered in shear flow to give sufficient interfacial area accessible to glucose. The reactivity of GOL clusters could be modulated on the basis of lipid composition of the membranes which affected the mode of interaction among liposomes through Ca 2+ . Part of GOL clusters could be separated from the reaction mixture by centrifugation, which would be advantageous for reusing liposomal catalysts. The liposome clusters can be the platforms to regulate the catalytic performance of glucose oxidase in the airlift.