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Fabrication of a Chitosan‐Coated Magnetic Nanobiocatalyst for Starch Hydrolysis
Author(s) -
Jain Manisha,
Sebatini Mariya,
Sharmila Govindasamy,
Muthukumaran Chandrasekaran,
Baskar Gurunathan,
Tamilarasan Krishnamurthi
Publication year - 2015
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201400493
Subject(s) - chitosan , hydrolysis , fourier transform infrared spectroscopy , starch , chemical engineering , chemistry , magnetic nanoparticles , nanoparticle , scanning electron microscope , amylase , response surface methodology , materials science , nuclear chemistry , chromatography , organic chemistry , composite material , engineering , enzyme
The preparation of chitosan‐coated magnetic nanoparticles (MNPs) and covalent immobilization of α ‐amylase for starch hydrolysis was investigated. Surface morphology, chemical composition, and structural characteristics of the MNPs were analyzed by scanning electron microscopy, energy dispersion spectrometry, and X‐ray diffractometry, respectively. Surface functional groups of MNPs, chitosan‐coated MNPs, and α ‐amylase‐immobilized MNPs were characterized by Fourier transform infrared spectroscopy. Response surface methodology based on three levels was implemented to optimize three immobilization conditions and a regression model was developed. α ‐Amylase‐immobilized MNPs provided better stability towards pH and temperature. The prepared thermostable nanobiocatalyst is well‐suited for industrial processes involving starch hydrolysis.