Premium
Discrete Modeling of Growth of Hairy Roots in a Mist Bioreactor
Author(s) -
Ranjan Ritu,
Katuri Sambasiva Rao,
Khanna Rajesh
Publication year - 2015
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201400402
Subject(s) - growth rate , bioreactor , nutrient , exponential growth , biological system , chemistry , environmental science , mathematics , biology , botany , mathematical analysis , geometry , organic chemistry
Abstract Hairy root culture is a promising route for large‐scale secondary metabolite production. A discrete model is developed to study the kinetic growth of these valuable roots in a reactor. The growth process is defined to occur in three sequential phases. The elongation rate is modeled as exponential growth with the growth coefficient being dependent on mass transfer coefficient, nutrient concentration difference, and distribution of nutrients in growth and sustenance requirements. Results indicate that the primary root growth is reduced by one‐fifth of its initial growth rate due to the branching process, and the growth of new branches is significantly faster than its primary root growth due to internal transport of nutrients. The model is successfully validated against experimental findings. Its integration with spatio‐temporal variation of nutrients in a reactor will be a crucial input to large‐scale production of hairy roots.