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Kinetic Model for Invertase‐Induced Sucrose Hydrolysis: Initial Time Lag
Author(s) -
Keramat Ali,
Kargari Ali,
Sohrabi† Morteza,
Mirshekar Hamed,
Sanaeepur Hamidreza
Publication year - 2017
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201400389
Subject(s) - invertase , lag time , hydrolysis , chemistry , kinetic energy , sucrose , lag , kinetics , time lag , thermodynamics , chromatography , biochemistry , biological system , computer science , biology , physics , computer network , quantum mechanics
The kinetics of sucrose hydrolysis by invertase was studied in order to find a comprehensive model for the reaction pathway and mechanism. First, three common models of Michaelis‐Menten (MM), substrate inhibition (S 2 ), and substrate clusters' inhibition (S 3 (I)) were investigated. The third model was found to better predict the initial sucrose concentration. Then, the S 3 (I) model was modified to cover the remaining pathway (S 3 (II)). Finally, a new comprehensive model (S 3 (III)) was evaluated, which in addition to what is considered in the two previously mentioned models (S 3 (I) and S 3 (II)) also involved the initial time lag. The model predictions showed an excellent agreement with the experimental data. The mean absolute error for the MM model is significantly reduced for the S 3 (III) model.