Kinetic Model for Invertase‐Induced Sucrose Hydrolysis: Initial Time Lag

The kinetics of sucrose hydrolysis by invertase was studied in order to find a comprehensive model for the reaction pathway and mechanism. First, three common models of Michaelis‐Menten (MM), substrate inhibition (S 2 ), and substrate clusters' inhibition (S 3 (I)) were investigated. The third model was found to better predict the initial sucrose concentration. Then, the S 3 (I) model was modified to cover the remaining pathway (S 3 (II)). Finally, a new comprehensive model (S 3 (III)) was evaluated, which in addition to what is considered in the two previously mentioned models (S 3 (I) and S 3 (II)) also involved the initial time lag. The model predictions showed an excellent agreement with the experimental data. The mean absolute error for the MM model is significantly reduced for the S 3 (III) model.