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Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies
Author(s) -
Eggersgluess Jan K.,
Richter Michael,
Dieterle Michael,
Strube Jochen
Publication year - 2014
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201300604
Subject(s) - chromatography , extraction (chemistry) , aqueous solution , process (computing) , stage (stratigraphy) , monoclonal antibody , chemistry , biomolecule , scale up , materials science , antibody , computer science , physics , organic chemistry , biology , biochemistry , paleontology , classical mechanics , immunology , operating system
Using monoclonal antibodies in a cell culture harvest as an example for complex biomolecules, a mini‐plant‐scale aqueous two‐phase purification process was studied. The results of this study indicate that antibodies can be concentrated and purified in a single extraction step employing a small phase ratio. Following the extraction step, a multi‐stage wash‐extraction for further purification was investigated. Starting at a test tube‐scale cross‐current wash, the process was advanced towards a continuous counter‐current mixer‐settler and column wash process. It was shown that a test tube cross‐current extraction operation can predict the multi‐stage purity reasonably well. The hydrodynamic process performance for the multi‐stage wash column was evaluated and related to the separation performance.