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Design Strategies for Integrated β ‐Galactosidase Purification Processes
Author(s) -
Lemes Ailton C.,
Machado Juliana Ribeiro,
Brites Marcela Lazzare,
Luccio Marco Di,
Kalil Susana Juliano
Publication year - 2014
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201300433
Subject(s) - diafiltration , ultrafiltration (renal) , chromatography , chemistry , downstream processing , ion exchange , expanded bed adsorption , microfiltration , membrane , biochemistry , ion , elution , organic chemistry
The operational conditions for an aqueous two‐phase system (ATPS) for β ‐galactosidase purification were optimized and applied to the design of a purification strategy as an alternative to the primary purification steps. The ATPS proved to be suitable for the recovery and primary enzyme purification. The purification process design developed by ATPS, diafiltration, ion exchange, and diafiltration/ultrafiltration was successful, yielding a more than tenfold purification. The purification strategy design resulted in a powerful integrated purification and recovery process, an evidence of the potential for a scale‐up of the β ‐galactosidase purification process.