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Partitioning Studies of Glutaminase in Polyethylene Glycol and Salt‐Based Aqueous Two‐Phase Systems
Author(s) -
Bolar S.,
Belur P. D.,
Iyyaswami R.
Publication year - 2013
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201200677
Subject(s) - polyethylene glycol , binodal , peg ratio , chemistry , aqueous solution , salt (chemistry) , glutaminase , phase (matter) , chromatography , organic chemistry , phase diagram , biochemistry , amino acid , finance , economics , glutamine
The partitioning behavior of glutaminase produced from Zygosaccharomyces rouxii in polyethylene glycol (PEG)‐salt aqueous two‐phase systems (ATPSs) was investigated. ATPSs comprising of different PEG‐salts were considered. Binodal data and tie lines generated for the selected systems were analyzed and correlated with Othmer‐Tobias and Bancroft equations. Effects of salt type, PEG molecular weight, concentrations of phase components, and tie line length on enzyme partitioning were evaluated.