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Efficient Chemo‐Enzymatic Epoxidation Using silcoat ‐Novozym ® 435: Characterizing the Multiphase System
Author(s) -
Bhattacharya S.,
Drews A.,
Lyagin E.,
Kraume M.,
AnsorgeSchumacher M. B.
Publication year - 2012
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201100640
Subject(s) - chemistry , catalysis , selectivity , candida antarctica , leaching (pedology) , enzyme , chromatography , phase (matter) , organic chemistry , nuclear chemistry , lipase , environmental science , soil science , soil water
Silicone composites of Novozym®435 ( silcoat ‐NZ435) demonstrate improved leaching and mechanical stability compared to native Novozym®435 (NZ435). Here, the application scope of this novel enzyme preparation has been broadened significantly by the transfer from single‐phase esterification to a complex chemo‐enzymatic epoxidation reaction in a three‐phase system. The reaction gave up to 80 % yields with almost 100 % selectivity in 50 h when operated in the absence of mass transfer limitations. Characterization of the system revealed that octanoic acid inhibited the reaction at concentrations around 6 M. The silcoat ‐NZ435 catalyst could be reused over five days with less than 50 % loss in activity when 5 M H 2 O 2 was present. H 2 O 2 was also identified as the component which deactivates the enzyme at high concentration (17.5 M) demanding spatial separation of the silcoat ‐NZ435 for optimal operation.