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Optimization and Performance of Silica‐Based Media for Industrial‐Scale Antibody Purification
Author(s) -
Miyahara H.,
Nakashima R.,
Inoue M.,
Katsuda T.,
Yamaji H.,
Katoh S.
Publication year - 2012
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201100245
Subject(s) - polyclonal antibodies , chromatography , ligand (biochemistry) , mass transfer , chemistry , silica gel , volume (thermodynamics) , materials science , high protein , chemical engineering , antibody , biochemistry , thermodynamics , engineering , receptor , immunology , biology , food science , physics
A silica‐based medium with optimized pore size, pore volume, and coupling density was prepared for protein A affinity chromatography, to be used for industrial purification of IgG. Performance and durability of the silica‐based medium were studied during repeated use and compared with other protein A media available on the market. The silica‐based medium provided high dynamic binding capacities for human polyclonal IgG in the superficial liquid velocity range from 150 to 720 cm h –1 because of a high mass transfer coefficient and high mechanical strength. The dynamic binding capacity and IgG recovery were high and varied only slightly during 50–150 cycles of repeated use. The release of protein A ligand was low.