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Preparation of Polyglycidylmethacrylate Macropore Beads and Application in Candida species 99–125 Lipase Immobilization
Author(s) -
Zhao R.,
Lu J.,
Tan T.
Publication year - 2011
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.201000263
Subject(s) - lipase , chemistry , suspension polymerization , immobilized enzyme , candida antarctica , toluene , polymer , chromatography , polymerization , thermal stability , nuclear chemistry , polymer chemistry , organic chemistry , enzyme
Polyglycidylmethacrylate beads with macroporous structure were synthesized by suspension polymerization and directly used for Candida species 99–125 lipase immobilization because of their rich surface epoxy groups. The properties of the synthesized polymer beads were characterized by scanning electron microscopy and FT‐IR spectroscopy. To increase the enzyme activity, divinyl benzene and toluene/heptane were used as cross‐linker and porogen, respectively, and the effects of cross‐linker content and porogen content on lipase immobilization were studied. After optimizing the synthesis conditions, the optimum lipase concentration for immobilization was determined as 10 mg mL –1 . The immobilized lipase showed a broader pH stability and higher temperature stability. The immobilized enzyme could be reused for ten batches with 62 % of residual activity.