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Model of a pH‐Based Potentiometric Biosensor Immobilizing Organophosphorus Hydrolase
Author(s) -
Lihong W.,
Lin Z.,
Jinxing H.,
Huanlin C.
Publication year - 2006
Publication title -
chemical engineering and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.403
H-Index - 81
eISSN - 1521-4125
pISSN - 0930-7516
DOI - 10.1002/ceat.200500281
Subject(s) - thiele modulus , potentiometric titration , chemistry , biosensor , immobilized enzyme , electrode , chromatography , buffer (optical fiber) , membrane , enzyme , organic chemistry , biochemistry , telecommunications , mass transfer , computer science
A model for predicting the steady‐state response of a pH‐based potentiometric biosensor immobilizing organophosphorus hydrolase (OPH) is developed. The model combines the processes of diffusion and enzymatic reaction in the membrane. Key factors influencing the system response, such as enzyme reaction order, Thiele modulus, and buffer concentration are discussed in detail. It is possible to obtain a good linear response by designing an enzyme electrode with a high enough Thiele modulus and by appropriately controlling added buffer concentrations. It is found that the model is in reasonable agreement with the experiment results.