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One Step Forward in Exploration of Class II Pyruvate Aldolases Nucleophile and Electrophile Substrate Specificity
Author(s) -
Gastaldi Cédric,
Ngahan Tagne Rolande,
Laurent Victor,
Hélaine Virgil,
Petit JeanLouis,
Traïkia Mounir,
Berardinis Véronique,
Lemaire Marielle,
GuérardHélaine Christine
Publication year - 2021
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.202100932
Subject(s) - nucleophile , aldolase a , electrophile , chemistry , substrate (aquarium) , combinatorial chemistry , stereochemistry , catalysis , organic chemistry , enzyme , biology , ecology
Originally named according to their strict nucleophile specificity towards pyruvate, some pyruvate aldolases were demonstrated to be able to convert other nucleophiles. This study illustrates that the nucleophile 2‐oxopentanoate successfully reacted with various aldehydes and even ketones. Unknown aldols with a 3S configuration were then isolated, characterized, and their stereochemistries could be set as either 4 R or 4 S by choosing the appropriate aldolase.