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Identification of Novel Unspecific Peroxygenase Chimeras and Unusual YfeX Axial Heme Ligand by a Versatile High‐Throughput GC‐MS Approach
Author(s) -
Knorrscheidt Anja,
Püllmann Pascal,
Schell Eugen,
Homann Dominik,
Freier Erik,
Weissenborn Martin J.
Publication year - 2020
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.202001467
Subject(s) - chimera (genetics) , chemistry , microtiter plate , mass spectrometry , chromatography , high throughput screening , heme , ligand (biochemistry) , combinatorial chemistry , biochemistry , receptor , enzyme , gene
The Front Cover depicts a multiple injection system for gas chromatography coupled with a mass spectrometer allowing the screening of enzymatically formed products. In their Full Paper, A. Knorrscheidt et al. described the method development for this microtiter plate screening system with the ability to inject 96 times within one chromatographical run enabling the simultaneous detection of a product, by‐product and internal standard. They applied their screening system to an unspecific peroxygenase chimera library in S. cerevisiae identifying novel peroxygenases for the hydroxylation of tetralin. More information can be found in the Full Paper by A. Knorrscheidt et al.