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Comparison of Enzymes Immobilised on Immobeads and Inclusion Bodies: A Case Study of a Trehalose Transferase
Author(s) -
Mestrom Luuk,
Marsden Stefan R.,
McMillan Duncan G. G.,
Schoevaart Rob,
Hagedoorn PeterLeon,
Hanefeld Ulf
Publication year - 2020
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.202000241
Subject(s) - chemistry , enzyme , trehalose , mcherry , biocatalysis , transferase , inclusion bodies , enzyme assay , combinatorial chemistry , biochemistry , immobilized enzyme , chromatography , catalysis , green fluorescent protein , recombinant dna , ionic liquid , gene
Abstract In this case study, we compare the performance of an enzyme immobilised using two different methods: i) as carrier‐free catalytically active inclusion bodies or ii) as carrier‐attached immobilised enzyme. To make this comparison we used a trehalose transferase from Thermoproteus uzoniensis fused to the fluorescent thermostable protein mCherry. The fusion of mCherry to trehalose transferase allowed direct spectrophotometric quantification and visualisation of the enzyme in both native and denatured states. The catalytically active inclusion bodies outperformed the immobilised enzyme in their simplicity of biocatalyst production resulting in high enzyme productivity. Enzyme immobilised on carrier materials showed a higher catalytic activity and a more robust performance under batch process conditions.