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Rapid, Heterogeneous Biocatalytic Hydrogenation and Deuteration in a Continuous Flow Reactor
Author(s) -
Thompson Lisa A.,
Rowbotham Jack S.,
Nicholson Jake H.,
Ramirez Miguel A.,
Zor Ceren,
Reeve Holly A.,
Grobert Nicole,
Vincent Kylie A.
Publication year - 2020
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.202000161
Subject(s) - biocatalysis , chemistry , flow chemistry , reductive amination , selectivity , enantiopure drug , catalysis , combinatorial chemistry , cofactor , continuous reactor , organic chemistry , reaction mechanism , enantioselective synthesis , enzyme
The high selectivity of biocatalysis offers a valuable method for greener, more efficient production of enantiopure molecules. Operating immobilised enzymes in flow reactors can improve the productivity and handling of biocatalysts, and using H 2 gas to drive redox enzymes bridges the gap to more traditional metal‐catalysed hydrogenation chemistry. Herein, we describe examples of H 2 ‐driven heterogeneous biocatalysis in flow employing enzymes immobilised on a carbon nanotube column, achieving near‐quantitative conversion in <5 min residence time. Cofactor recycling is carried out in‐situ using H 2 gas as a clean reductant, in a completely atom‐efficient process. The flow system is demonstrated for cofactor conversion, reductive amination and ketone reduction, and then extended to biocatalytic deuteration for the selective production of isotopically labelled chemicals.