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Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid
Author(s) -
Xu Xiaomin,
But Andrada,
Wever Ron,
Hollmann Frank
Publication year - 2020
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201902194
Subject(s) - decarboxylation , oxidative decarboxylation , chemistry , catalysis , nitrile , oxidative phosphorylation , organic chemistry , glutamate receptor , biochemistry , receptor
The chemoenzymatic oxidative decarboxylation of glutamic acid to the corresponding nitrile using the vanadium chloroperoxidase from Curvularia inaequalis ( Ci VCPO) as HOBr generation catalysts has been investigated. Product inhibition was identified as major limitation. Nevertheless, 1630000 turnovers and k cat of 75 s −1 were achieved using 100 mM glutamate. The semi‐preparative enzymatic oxidative decarboxylation of glutamate was also demonstrated.