Premium
Development of a High‐Pressure Reactor Based on Liquid‐Flow Pressurisation to Facilitate Enzymatic Hydroxylation of Gaseous Alkanes
Author(s) -
Ariyasu Shinya,
Kodama Yusaku,
Kasai Chie,
Cong Zhiqi,
Stanfield Joshua Kyle,
Aiba Yuichiro,
Watanabe Yoshihito,
Shoji Osami
Publication year - 2019
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201901323
Subject(s) - hydroxylation , autoclave , chemistry , propane , substrate (aquarium) , high pressure , chemical engineering , flow chemistry , organic chemistry , chromatography , catalysis , enzyme , oceanography , engineering physics , engineering , geology
A new type of high‐pressure reactor based on liquid‐flow pressurisation using a HPLC pump has been developed. This high‐pressure reactor allows the easy and safe performance of reactions with gaseous alkanes under high‐pressures up to 10 MPa (100 atm), without the need for high‐pressure gas cylinders. The amount of substrate gas required for a single reaction is very small compared with reactions using a conventional autoclave, which, when using expensive substrate gasses, such as 13 C‐labelled ethane, becomes critical. Employing this high‐pressure reactor in conjunction with cytochrome P450BM3 and the assistance of decoy molecules, the direct hydroxylation of gaseous alkanes was drastically improved. At 5 MPa the TOF of propane hydroxylation increased 10‐fold, reaching 2200 min −1 . Hydroxylation of ethane was also substantially accelerated at 5 MPa, reaching a TOF of 28 min −1 .