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Enzyme Immobilization in MOF‐derived Porous NiO with Hierarchical Structure: An Efficient and Stable Enzymatic Reactor
Author(s) -
Gao Xia,
Ding Yu,
Sheng Yude,
Hu Mancheng,
Zhai Quanguo,
Li Shuni,
Jiang Yucheng,
Chen Yu
Publication year - 2019
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201900611
Subject(s) - chemistry , horseradish peroxidase , immobilized enzyme , substrate (aquarium) , catalysis , 2,4 dichlorophenol , thermal stability , enzyme , chromatography , metal organic framework , enzyme catalysis , biocatalysis , chemical engineering , nuclear chemistry , organic chemistry , adsorption , reaction mechanism , engineering , oceanography , biology , bacteria , genetics , geology
MOF‐derived porous NiO with hierarchical structure (MHNiO) was prepared based on thermolysis of Ni metal organic framework (Ni‐MOF), which was used as carrier for the immobilization of horseradish peroxidase (HRP) and cytochrome C (Cyt c). The pore size of MHNiO was tuned as 11.8 nm to match the size of free enzyme so as to depress the aggregation of the enzymes in the pore. Meanwhile, the hierarchical structure allowed the substrates concentrated in the vicinity of the enzymes. The obtained enzymatic reactors exhibited better thermal stability, storage stability and reusability. For example, over 83 % of its original activity after 1 h incubation at 70 °C could be remained compared to 18.1 % or 38.4 % remained activity of free HRP and Cyt c, respectively; After 12 cycles of use, over 53 % of original catalytic activity of both the enzymatic reactor could be maintain. The enzymatic kinetic data ( K m , V max , k cat ) and thermodynamic data ( K a , Δ H , Δ S and Δ G ) indicated that the improved catalytic performance was attributed to the affinity, selectivity and binding of enzyme to substrate. The two enzymatic reactors could be applied in the fast degradation of 2,4‐dichlorophenol and rifaximin in artificial wastewater, a complete degradation of 2 mg ⋅ mL −1 of 2,4‐dichlorophenol or 20 μg ⋅ mL −1 of rifaximin was achieved in only 20 min.