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Glutamate as an Efficient Amine Donor for the Synthesis of Chiral β‐ and γ‐Amino Acids Using Transaminase
Author(s) -
Kim GeonHee,
Jeon Hyunwoo,
Khobragade Taresh P.,
Patil Mahesh D.,
Sung Sihyong,
Yoon Sanghan,
Won Yumi,
Sarak Sharad,
Yun Hyungdon
Publication year - 2019
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201802048
Subject(s) - enantiopure drug , transaminase , amine gas treating , chemistry , amino acid , formate dehydrogenase , glutamate dehydrogenase , enantiomeric excess , formate , enantiomer , glutamate receptor , organic chemistry , combinatorial chemistry , enzyme , enantioselective synthesis , biochemistry , catalysis , receptor
A recyclable glutamate amine donor system employing transaminase (TA), glutamate dehydrogenase (GluDH) and mutant formate dehydrogenase (FDHm) was developed, wherein amine donor Glu was regenerated using GluDH and thereby circumvented the inhibition of TA by α‐ketoglutarate. Various enantiopure β‐, γ‐amino acids, and amines were successfully synthesized with high conversions and excellent enantiomeric excess using this system.