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Upgrading of Biomass Monosaccharides by Immobilized Glucose Dehydrogenase and Xylose Dehydrogenase
Author(s) -
Zdarta Jakub,
Pinelo Manuel,
Jesionowski Teofil,
Meyer Anne S.
Publication year - 2018
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201801335
Subject(s) - chemistry , xylose , monosaccharide , immobilized enzyme , mesoporous silica , gluconic acid , enzyme , dehydrogenase , chromatography , nuclear chemistry , biochemistry , catalysis , fermentation , mesoporous material
Direct upgrading and separation of the monosaccharides from biomass liquors is an overlooked area. In this work we demonstrate enzymatic production of gluconic acid and xylonic acid from glucose and xylose present in pretreated birchwood liquor by glucose dehydrogenase (GDH, EC 1.1.1.47) and xylose dehydrogenase (XDH, EC 1.1.1.175), respectively. The biocatalytic conversions were compared using two different kinds of silica support materials (silica nanoparticles (nanoSiO 2 ) and porous silica particles with hexagonal pores (SBA 15 silica) for enzyme immobilization. Upon immobilization, both enzymes showed significant improvement in their thermal stability and robustness at alkaline pH and exhibited over 50 % activity even at pH 10 and 60 °C on both immobilization matrices. When compared to free enzymes at 45 °C, GDH immobilized on nanoSiO 2 and SBA silica displayed a 4.5 and 7.25 fold increase in half‐life, respectively, whilst XDH immobilized on nanoSiO 2 and SBA showed a 4.7 and 9.5 fold improvement in half‐life, respectively. Additionally, after five reaction cycles both nanoSiO 2 GDH and nanoSiO 2 XDH retained more than 40 % activity and GDH and XDH immobilized on SBA silica maintained around 50 % of their initial activity resulting in about 1.5–1.6 fold increase in biocatalytic productivity compared to the free enzymes.