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Streptavidin–Enzyme Linked Aggregates for the One‐Step Assembly and Purification of Enzyme Cascades
Author(s) -
Mallin Hendrik,
Ward Thomas R.
Publication year - 2018
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201800162
Subject(s) - streptavidin , chemistry , enantiopure drug , enzyme , combinatorial chemistry , covalent bond , matrix (chemical analysis) , peptide , biocatalysis , lactate dehydrogenase , biochemistry , chromatography , organic chemistry , biotin , catalysis , enantioselective synthesis , ionic liquid
Herein, we report enzyme aggregates assembled around covalently cross‐linked streptavidin tetramers. The streptavidin oligomeric matrix (Sav Matrix ) is produced by using SpyTag/SpyCatch technology and binds tightly to fusion proteins bearing a streptavidin‐binding peptide (SBP). Fusing the SBPs to different enzymes leads to precipitation of the streptavidin–enzyme aggregates upon mixing the complementary components. This straightforward strategy can be applied to crude cell‐free extracts, allowing the one‐step assembly and purification of catalytically active aggregates. Enzyme cascade assemblies can be produced upon adding different SBP‐fused enzymes to the Sav Matrix . The reaction rate for lactate dehydrogenase (LDH) is improved tenfold (compared with the soluble enzyme) upon precipitation with the Sav Matrix from crude cell‐free extracts. Additionally, the kinetic parameters are improved. A cascade combining a transaminase with LDH for the synthesis of enantiopure amines from prochiral ketones displays nearly threefold rate enhancement for the synthesis of ( R )‐α‐methylbenzylamine compared with the free enzymes in solution.