Lipase Encapsulation onto ZIF‐8: A Comparison between Biocatalysts Obtained at Low and High Zinc/2‐Methylimidazole Molar Ratio in Aqueous Medium | Zendy

Pitzalis Federica | Zendy; Carucci Cristina | Zendy; Naseri Maryam | Zendy; Fotouhi Lida | Zendy; Magner Edmond | Zendy; Salis Andrea | Zendy

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Lipase Encapsulation onto ZIF‐8: A Comparison between Biocatalysts Obtained at Low and High Zinc/2‐Methylimidazole Molar Ratio in Aqueous Medium

Author(s) -

Pitzalis Federica,

Carucci Cristina,

Naseri Maryam,

Fotouhi Lida,

Magner Edmond,

Salis Andrea

Publication year - 2018

Publication title -

chemcatchem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.497

H-Index - 106

eISSN - 1867-3899

pISSN - 1867-3880

DOI - 10.1002/cctc.201701984

Subject(s) - rhizomucor miehei , lipase , chemistry , imidazolate , aqueous solution , zinc , biocatalysis , molar ratio , nuclear chemistry , pseudomonas fluorescens , zeolitic imidazolate framework , catalysis , metal organic framework , organic chemistry , triacylglycerol lipase , enzyme , reaction mechanism , adsorption , biology , bacteria , genetics

Lipase AK from Pseudomonas fluorescens and Lipase RM from Rhizomucor miehei were encapsulated into a zeolite imidazolate framework (ZIF‐8) by a “one‐pot” synthesis to obtain AK@ZIF‐8 and RM@ZIF‐8 biocatalysts. The effect of a high (1:40) and low (1:4) Zn/2‐methylimidazole molar ratio on the biocatalysts synthesis was investigated. The different Zn/ligand (L) ratios affected both the surface area, the loading, and the specific activity of the biocatalysts. Samples synthesized by using a high Zn/L ratio had high values of surface area whereas those obtained by using a low Zn/L ratio had higher loadings and specific activities. The decrease of pH (from 11.6 to 9.4) during the synthesis at high Zn/L ratio produced ZIF‐8 samples with features similar to those observed for low Zn/L ratio samples. The low Zn/L (1:4) ratio AK@ZIF‐8 biocatalyst retained 99 % activity after storage for 15 days at 5 °C and 40 % activity after five reaction cycles.

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