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Lipase Encapsulation onto ZIF‐8: A Comparison between Biocatalysts Obtained at Low and High Zinc/2‐Methylimidazole Molar Ratio in Aqueous Medium
Author(s) -
Pitzalis Federica,
Carucci Cristina,
Naseri Maryam,
Fotouhi Lida,
Magner Edmond,
Salis Andrea
Publication year - 2018
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201701984
Subject(s) - rhizomucor miehei , lipase , chemistry , imidazolate , aqueous solution , zinc , biocatalysis , molar ratio , nuclear chemistry , pseudomonas fluorescens , zeolitic imidazolate framework , catalysis , metal organic framework , organic chemistry , triacylglycerol lipase , enzyme , reaction mechanism , adsorption , biology , bacteria , genetics
Lipase AK from Pseudomonas fluorescens and Lipase RM from Rhizomucor miehei were encapsulated into a zeolite imidazolate framework (ZIF‐8) by a “one‐pot” synthesis to obtain AK@ZIF‐8 and RM@ZIF‐8 biocatalysts. The effect of a high (1:40) and low (1:4) Zn/2‐methylimidazole molar ratio on the biocatalysts synthesis was investigated. The different Zn/ligand (L) ratios affected both the surface area, the loading, and the specific activity of the biocatalysts. Samples synthesized by using a high Zn/L ratio had high values of surface area whereas those obtained by using a low Zn/L ratio had higher loadings and specific activities. The decrease of pH (from 11.6 to 9.4) during the synthesis at high Zn/L ratio produced ZIF‐8 samples with features similar to those observed for low Zn/L ratio samples. The low Zn/L (1:4) ratio AK@ZIF‐8 biocatalyst retained 99 % activity after storage for 15 days at 5 °C and 40 % activity after five reaction cycles.