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Efficient Biocatalytic Reductive Aminations by Extending the Imine Reductase Toolbox
Author(s) -
Roiban GheorgheDoru,
Kern Marcelo,
Liu Zhi,
Hyslop Julia,
Tey Pei Lyn,
Levine Matthew S.,
Jordan Lydia S.,
Brown Kristin K.,
Hadi Timin,
Ihnken Leigh Anne F.,
Brown Murray J. B.
Publication year - 2017
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201701379
Subject(s) - imine , reductive amination , chemistry , combinatorial chemistry , biocatalysis , amination , organic chemistry , catalysis , reaction mechanism
Chiral secondary and tertiary amines are ubiquitous in pharmaceutical, fine, and specialty chemicals, but their synthesis typically suffers from significant sustainability and selectivity challenges. Biocatalytic alternatives, such as enzyme‐catalyzed reductive amination, offer several advantages over traditional chemistry, but industrial applicability has not yet been demonstrated. Herein, we report the use of cell lysates expressing imine reductases operating at 1:1 stoichiometry for a variety of amines and carbonyls. A collection of biocatalysts with diversity in coverage of small molecules and direct industrial applicability is presented.