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Bootstrapped Biocatalysis: Biofilm‐Derived Materials as Reversibly Functionalizable Multienzyme Surfaces
Author(s) -
Nussbaumer Martin G.,
Nguyen Peter Q.,
Tay Pei K. R.,
Naydich Alexander,
Hysi Erisa,
Botyanszki Zsofia,
Joshi Neel S.
Publication year - 2017
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201701221
Subject(s) - biocatalysis , biofilm , cofactor , chemistry , substrate (aquarium) , enzyme , catalysis , immobilized enzyme , synthetic biology , combinatorial chemistry , nanotechnology , materials science , organic chemistry , reaction mechanism , biology , bacteria , computational biology , ecology , genetics
Cell‐free biocatalysis systems offer many benefits for chemical manufacturing, but their widespread applicability is hindered by high costs associated with enzyme purification, modification, and immobilization on solid substrates, in addition to the cost of the material substrates themselves. Herein, we report a “bootstrapped” biocatalysis substrate material that is produced directly in bacterial culture and is derived from biofilm matrix proteins, which self‐assemble into a nanofibrous mesh. We demonstrate that this material can simultaneously purify and immobilize multiple enzymes site specifically and directly from crude cell lysates by using a panel of genetically programmed, mutually orthogonal conjugation domains. We further demonstrate the utility of the technique in a bienzymatic stereoselective reduction coupled with a cofactor recycling scheme. The domains allow for several cycles of selective removal and replacement of enzymes under mild conditions to regenerate the catalyst system.