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Water as Cosolvent: Nonviscous Deep Eutectic Solvents for Efficient Lipase‐Catalyzed Esterifications
Author(s) -
Guajardo Nadia,
Domínguez de María Pablo,
Ahumada Katherine,
Schrebler Rodrigo A.,
RamírezTagle Rodrigo,
Crespo Fernando A.,
Carlesi Carlos
Publication year - 2017
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201601575
Subject(s) - choline chloride , deep eutectic solvent , chemistry , glycerol , lipase , candida antarctica , ethylene glycol , solvent , urea , biocatalysis , nuclear chemistry , catalysis , organic chemistry , chloride , substrate (aquarium) , eutectic system , enzyme , ionic liquid , alloy , oceanography , geology
The use of deep eutectic solvent (DES)/water mixtures were explored for the selective enzymatic synthesis of α‐monobenzoate glycerol (α‐MBG) from glycerol and benzoic acid as substrates. Experiments were performed with four DES, three of them containing choline chloride (ChCl), combined with urea (URA), glycerol (GLY), and ethylene glycol (ETA) (in all cases ChCl/HBD 1:2 mol ratio), and another one formed with methylammonium chloride and glycerol (MA/GLY 1:3 mol ratio). The best conversions (99 %) were achieved with immobilized lipase B from Candida antarctica (CAL‐B) when ChCl/GLY was used as the solvent and the substrate at the same time. The use of water as a cosolvent (8 % v / v ) led to a significant decrease in the viscosity of the DES, and full conversions were then reached. Reusability studies of the biocatalyst revealed a 37 % decrease in activity after the first batch, but the activity remained mostly constant for the rest of the cycles.