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Water Splitting for High‐Yield Hydrogen Production Energized by Biomass Xylooligosaccharides Catalyzed by an Enzyme Cocktail
Author(s) -
Moustafa Hanan M. A.,
Kim EuiJin,
Zhu Zhiguang,
Wu ChangHao,
Zaghloul Taha I.,
Adams Michael W. W.,
Zhang Y.H. Percival
Publication year - 2016
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201600772
Subject(s) - xylose , chemistry , catalysis , yield (engineering) , enzyme , biomass (ecology) , hydrogen production , phosphate , hydrogen , isomerase , enzyme catalysis , biochemistry , organic chemistry , fermentation , materials science , biology , agronomy , metallurgy
Green hydrogen production through water splitting at low temperatures is highly desired for hydrogen economy. Herein, we demonstrate an in vitro non‐natural enzymatic pathway to utilize the chemical energy stored in xylooligosaccharides from biomass to split water to produce a nearly theoretical yield of H 2 (i.e., ≈9.5 H 2 per xylose plus water). This pathway was constructed on the basis of the novel activities of phosphopentomutase catalyzing the conversion of d ‐xylose 1‐phosphate into d ‐xylose 5‐phosphate and of ribose 5‐phosphate isomerase catalyzing the conversions of d ‐xylose 5‐phosphate and d ‐xylulose 5‐phosphate. This study suggests that the discovery of novel promiscuous enzyme activities is important to implement complicated biotransformations catalyzed by synthetic enzymatic pathways.