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Design of Artificial Metabolisms in Layered Nanomaterials for the Enzymatic Synthesis of Phosphorylated Sugars
Author(s) -
Mahdi Rima,
GuérardHélaine Christine,
Prévot Vanessa,
de Berardinis Véronique,
Forano Claude,
Lemaire Marielle
Publication year - 2015
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201500606
Subject(s) - dhap , dihydroxyacetone phosphate , aldolase a , dihydroxyacetone , chemistry , aldol reaction , biocatalysis , enzyme catalysis , combinatorial chemistry , nanoreactor , enzyme , catalysis , organic chemistry , reaction mechanism , glycerol
Biohybrid nanoreactors operating in one‐pot cascade reactions were designed by co‐immobilization of enzymes in an inorganic layered matrix, namely, layered double hydroxides. These biohybrid systems were devoted to prepare dihydroxyacetone phosphate (DHAP) and phosphorylated sugars through stereoselective C−C bond formation. In the first system, two kinases were exploited for the in situ generation of DHAP. Increasing the complexity, the second nano‐bioreactor combined up to four enzymes to lead to d ‐fructose‐6‐phosphate from the aldol‐catalyzed addition of dihydroxyacetone to d ‐glyceraldehyde‐3‐phosphate generated in situ from DHAP. The biohybrid catalyst showed the same reaction rate as that of the free enzymes and was reusable.