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Performance of Recombinant‐Whole‐Cell‐Catalyzed Reductions in Deep‐Eutectic‐Solvent–Aqueous‐Media Mixtures
Author(s) -
Müller Christoph R.,
Lavandera Iván,
GotorFernández Vicente,
Domínguez de María Pablo
Publication year - 2015
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201500428
Subject(s) - deep eutectic solvent , biocatalysis , chemistry , alcohol dehydrogenase , substrate (aquarium) , eutectic system , enantiomeric excess , solvent , aqueous solution , biotransformation , catalysis , organic chemistry , combinatorial chemistry , enzyme , enantioselective synthesis , reaction mechanism , alloy , oceanography , geology
Deep‐eutectic solvents (DES) are cost‐effective, nonhazardous solvents that may be used in biocatalysis as nonconventional media that enable biotransformations with industrially sound high substrate loadings. Based on promising prognoses, this paper explores successfully the use of whole cells overexpressing oxidoreductases as biocatalysts for the stereoselective reduction of ketones in different DES–aqueous‐media solutions. Enzymes like Ralstonia sp. alcohol dehydrogenase (ADH) and horse liver ADH, overexpressed in Escherichia coli , remain active at different DES proportions (for example, DES:buffer, 80:20 v/v) to enable nonconventional biotransformations. Furthermore, the enantiomeric excesses obtained for a broad range of aromatic substrates increase significantly if DES is added, which provides a useful tool for combining high substrate loadings and improved enzymatic selectivities while working with nonhazardous solvents. Different DES are successfully employed, which gives hints of the potential that these emerging solvents may have.