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Cover Picture: Whole‐Cell One‐Pot Biosynthesis of Azelaic Acid (ChemCatChem 4/2014)
Author(s) -
Otte Konrad B.,
Kittelberger Jens,
Kirtz Marko,
Nestl Bettina M.,
Hauer Bernhard
Publication year - 2014
Publication title -
chemcatchem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201490020
Subject(s) - azelaic acid , bioconversion , linoleic acid , biosynthesis , biochemistry , enzyme , dehydrogenase , chemistry , lipoxygenase , escherichia coli , fatty acid , fermentation , gene
Bacterial ozonolysis The cover picture shows the Escherichia coli whole‐cell‐based biosynthesis of azelaic acid from linoleic acid starting material, which is enabled by a three‐step enzymatic cascade reaction. In their Full Paper on p. 1003 ff., K. B. Otte et al. investigate the simultaneous lipoxygenase and hydroperoxide lyase‐catalyzed oxidation and cleavage reaction of linoleic acid, which is derived from the plant oxylipin metabolism. Adjustment of the in vivo expression levels of these enzymes with respect to the lipoxygenase activation behavior results in the development of a dual expression system. In combination with an additional endogenous E. coli dehydrogenase, the artificial pathway for the bioconversion of linoleic acid into azelaic acid is completed.