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Whole‐Cell Biocatalysis in Deep‐Eutectic‐Solvents/Aqueous Mixtures
Author(s) -
Maugeri Zaira,
Domínguez de María Pablo
Publication year - 2014
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201400077
Subject(s) - biocatalysis , choline chloride , enantioselective synthesis , chemistry , eutectic system , aqueous solution , ketone , deep eutectic solvent , glycerol , green chemistry , yeast , enantiomer , enantiomeric excess , organic chemistry , solvent , ionic liquid , catalysis , biochemistry , alloy
Whole‐cell biocatalysis with the use of baker’s yeast is demonstrated in different mixtures of water with deep eutectic solvents (DESs; choline chloride/glycerol, 1:2 mol/mol). Enantioselective ketone reduction is observed for long reaction times (>200 h), which suggests that the whole cells remain stable in these neoteric solvents. By changing the proportion of the DES added, a complete inversion of enantioselectivity is observed, from approximately 95 % enantiomeric excess ( ee ) ( S ) in pure water to approximately 95 % ee ( R ) in the pure DES. Presumably, some ( S )‐oxidoreductases present in baker’s yeast are inhibited by DESs.