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An Enzymatic Toolbox for Cascade Reactions: A Showcase for an In Vivo Redox Sequence in Asymmetric Synthesis
Author(s) -
Oberleitner Nikolin,
Peters Christin,
Muschiol Jan,
Kadow Maria,
Saß Stefan,
Bayer Thomas,
Schaaf Patricia,
Iqbal Naseem,
Rudroff Florian,
Mihovilovic Marko D.,
Bornscheuer Uwe T.
Publication year - 2013
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201300604
Subject(s) - biocatalysis , alcohol dehydrogenase , cascade , chemistry , monooxygenase , redox , cascade reaction , enzyme , combinatorial chemistry , kinetic resolution , enantioselective synthesis , stereochemistry , biochemistry , organic chemistry , catalysis , cytochrome p450 , reaction mechanism , chromatography
Joined forces: Alcohol dehydrogenase, enoate reductase, and Baeyer–Villiger monooxygenase are combined in a cascade reaction by coexpression in E. coli to have a recombinant whole‐cell biocatalyst. Such an artificial metabolic “mini”‐pathway provides access to functionalized chiral compounds in high yields and optical purities as exemplified for kinetic resolutions, desymmetrizations, and regiodivergent biotransformations.