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Designing Functionalized Mesoporous Materials for Enzyme Immobilization: Locating Enzymes by Using Advanced TEM Techniques
Author(s) -
Mayoral Alvaro,
Arenal Raul,
Gascón Victoria,
MárquezÁlvarez Carlos,
Blanco Rosa M.,
Díaz Isabel
Publication year - 2013
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201200737
Subject(s) - mesoporous material , materials science , immobilized enzyme , surface modification , mesoporous organosilica , lipase , nanotechnology , mesoporous silica , laccase , biocatalysis , polymer , matrix (chemical analysis) , chemical engineering , catalysis , chemistry , organic chemistry , enzyme , composite material , engineering , ionic liquid
One of the widely accepted uses of ordered mesoporous materials is as supports of enzymes for biotechnological applications. Enzymes have been trapped, anchored, or encapsulated in organized porous networks of the mesoporous range (2–50 nm). The reactivity of the surface of mesoporous materials has enabled the synthesis of various supports by using different forces for the immobilization process. To design catalysts for specific applications, we have developed functionalized mesoporous materials with tunable hydrophobicity for the immobilization of lipase. More recently, we moved to the immobilization of laccase with amino‐functionalized ordered mesoporous materials. In this case, it is required to use pore expanders along with optimized functionalization techniques. Advanced TEM techniques have been applied to locate not only the functional groups but also the macromolecules inside the silica matrix.