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Dye Degradation by Layer‐by‐Layer Immobilised Peroxidase/Redox Mediator Systems
Author(s) -
Saladino Raffaele,
Guazzaroni Melissa,
Crestini Claudia,
Crucianelli Marcello
Publication year - 2013
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201200660
Subject(s) - horseradish peroxidase , chemistry , catalysis , redox , peroxidase , quinoline , nuclear chemistry , layer by layer , alcohol , combinatorial chemistry , organic chemistry , layer (electronics) , enzyme
Horseradish peroxidase (HRP) was immobilised on Eupergit C 250 L resin coated with poly‐electrolytes, or by entrapment inside pre‐formed layer‐by‐layer (LbL) micro‐capsules of poly‐electrolytes. In these systems, namely HRP/E‐LbL, HRPm/LbL and HRPm/LbLp, the native enzyme retained its catalytic activity. Immobilised HRP showed a significant activity in the oxidation of selected azo, quinoline and fluorone dyes with H 2 O 2 as the primary oxidant under mild experimental conditions, and HRPm/LbL was the best catalyst. A comparison between the catalytic efficiency of different redox mediators for HRP activity was made by using 1‐hydroxybenzotriazole (HOBt), violuric acid (VLA) and veratrylic alcohol (VA). As a general trend, azo dyes were degraded in higher yields, and HOBt was the best mediator for the oxidation. The degradation yield increased on increasing the reaction time and reached the highest value after 12 h, which is comparable with that observed for native HRP. Notably, HRPm/LbL retained its catalytic activity for more runs.