Premium
Synthesis of Aldehydes by Layer‐by‐Layer Immobilized Laccases in the Presence of Redox Mediators
Author(s) -
Guazzaroni Melissa,
Bozzini Tiziana,
Saladino Raffaele
Publication year - 2012
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201200330
Subject(s) - laccase , chemistry , immobilized enzyme , trametes versicolor , abts , catalysis , redox , layer by layer , biocatalysis , layer (electronics) , organic chemistry , combinatorial chemistry , chromatography , nuclear chemistry , enzyme , reaction mechanism , antioxidant , dpph
Laccase from Trametes versicolor was immobilized on Eupergit ® C250L and alumina particles. The immobilized enzyme was protected by coating with oppositely charged polyelectrolytes by means of the Layer‐by‐Layer (LbL) technique. Enzymatic assay showed that immobilized laccase was more stable than free enzyme at different pH and temperatures without loss of catalytic efficiency. Immobilized laccase based catalysts were efficiently used for the selective oxidation of alcohols to aldehydes in the presence of redox mediators such as ABTS (2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulphonic acid), HBT (1‐hydroxybenzotriazole), VLA (violuric acid) and TEMPO (2,2,6,6‐tetramethyl‐1‐piperidinyloxy free radical)