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Directed Evolution of P 450 BM 3 into a p ‐Xylene Hydroxylase
Author(s) -
Dennig Alexander,
Marienhagen Jan,
Ruff Anna Joelle,
Guddat Lukas,
Schwaneberg Ulrich
Publication year - 2012
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201200092
Subject(s) - reuse , catalysis , enzyme kinetics , production (economics) , selectivity , chemistry , enzyme , xylene , combinatorial chemistry , computer science , organic chemistry , active site , toluene , engineering , microeconomics , waste management , economics
Reduce, reuse, reengineer! 2,5‐Dimethylphenol is of high interest for the production of temperature stable polymers (>500 K) and as a building block for “next‐generation” plastics. The presented reengineered P 450 BM 3 variant (R 47 S/Y 51 W/I 401 M) has a good catalytic activity ( k cat =1950 min −1 ), excellent coupling efficiency (66 %), and selectivity (>98 %) for 2,5‐dimethylphenol production.