Premium
Multienzyme Whole‐Cell In Situ Biocatalysis for the Production of Flaviolin in Permeabilized Cells of Escherichia coli
Author(s) -
Krauser Steffen,
Kiefer Patrick,
Heinzle Elmar
Publication year - 2012
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201100351
Subject(s) - biocatalysis , escherichia coli , synthetic biology , biochemical engineering , metabolite , biochemistry , chemistry , computational biology , metabolic engineering , combinatorial chemistry , database , enzyme , biology , microbiology and biotechnology , nanotechnology , computer science , catalysis , gene , materials science , reaction mechanism , engineering
Serial conversion: Biocatalysis is applied to secondary metabolite synthesis through a new strategy. A one‐pot multienzyme cascade reaction is performed with tailored permeabilized whole Escherichia coli cells in a well‐defined environment. Acetate (C 2 ) is converted to flaviolin (C 10 ) through three serial biocatalytic conversion steps under the consumption of adenosine 5'‐triphosphate (ATP).